Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 63, Issue 9, Pages 871-874Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S106193480809013X
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- International Science and Technology Center [1892.2]
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Conditions were selected for the separation of the herbicide glyphosate (N-(phosphonomethyl)glycine) and products of its microbiological utilization as N-acylated derivatives by ion-exchange liquid chromatography. The order of the elution of compounds on a Repro-Gel H column with UV detection correlates with their structures. The detection limits of the derivatives (wavelength 210 nm) are as follows (ng): glyphosate, 30; glycine and sarcosine, 20 and 43, respectively; aminomethylphosphonic acid, 45. The detection limit of methylphosphonic acid is 14 mu g. Glyphosate and its biodegradation products were separated by thin-layer chromatography on plates with silica gel in the system isopropanol-5% aqueous ammonia solution (1: 1).
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