Study of thiol-disulfide equilibrium in the whole blood and its fractions by stripping voltammetric titration

A stripping voltammetric signal is used as the source of information in the new version of electrochemical analysis, stripping voltammetric titration (SVT). The reaction RSH + AgNO3 = AgSR + HNO3 is used to substantiate the method. A procedure is developed to study the thiol-disulfide equilibrium in the whole blood and its fractions (hemolysate, plasma, and erythrocyte mass). Disulfides are reduced by sodium sulfite before their determination. The accuracy of the results is verified in the analysis of model solutions and using the added-found method. A version of rapid analysis from a single aliquot portion of a test sample is proposed.