Journal
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
Volume 26, Issue 6, Pages 1175-1177Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ja00010a
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Funding
- National Natural Science Foundation of China [21035006, 20775062]
- 973 National Basic Research Program [2009CB421605]
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In this proof-of-concept study, a novel dual-labelling strategy for conjugating -SH and -NH2 of a peptide was developed, in which an element tag (1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid-10-maleimidoethylacetamide loaded with europium) and a fluorescent tag (fluorescein isothiocycanate) were employed. Its feasibility was demonstrated using HPLC-UV/ESI-MS for evaluating labelling-efficiency and HPLC-ICPMS with Eu-153 isotope dilution as well as CE-LIF for the determination of peptides.
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