Journal
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
Volume 25, Issue 3, Pages 260-268Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b921770c
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Funding
- NSERC Canada
- National Institutes of Health [NIH] [R01-GM076127]
- Genome Canada
- Ontario Genomics Institute
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076127] Funding Source: NIH RePORTER
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We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.
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