Journal
JOURNAL OF ALZHEIMERS DISEASE
Volume 38, Issue 2, Pages 281-293Publisher
IOS PRESS
DOI: 10.3233/JAD-131070
Keywords
Alzheimer's disease; amyloid-beta toxicity; bindarit; CCL2; neuroinflammation
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Funding
- Italian Ministry of Higher Education and Scientific Research, IT (FIRB) [RBAP10L8TY 004]
- Fondazione Roma/Banca di Roma
- Fondazione Enrico and Enrica Sovena, Rome, Italy
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One of the hallmarks of Alzheimer's disease (AD), the most common age-related neurodegenerative pathology, is the abnormal extracellular deposition of neurotoxic amyloid-beta (A beta) peptides that accumulate in senile plaques. A beta aggregates are toxic to neurons and are thought to contribute to neuronal loss. Evidence indicates that inflammation is involved in the pathophysiology of AD, and activation of glial cells by a variety of factors, including A beta, appears to be a central event. Among molecules produced during inflammation associated with neuronal death, CCL2, also known as monocyte chemotactic protein-1 (MCP-1), seems to be particularly important. Indeed, CCL2 levels are higher in the cerebrospinal fluid of patients with AD than in controls. In the present study, we demonstrated the protective effect of bindarit (which inhibits CCL2 synthesis) against both A beta(25-35) and A beta(1-42)-induced toxicity in primary mixed neural cultures. Bindarit (30-500 mu M) reversed cell death induced by A beta in a dose-dependent manner and reduced the transcription and release of CCL2 by astrocytes after A beta treatment, as revealed by qRT-PCR, ELISA, and immunofluorescence staining. Astroglial activation and CCL2 release was induced by ATP released by damaged neurons through interaction with P2X(7) receptors present on astrocyte surface. CCL2, interacting with its cognate receptor CCR2, present on neuron surface, strongly contributes to the toxic activity of A beta. Bindarit was able to disconnect this neuro-glial interaction. Our results demonstrate the ability of bindarit to inhibit A beta-induced neuronal death and suggest the potential role of CCL2 inhibitors in the treatment of neuroinflammatory/neurodegenerative diseases.
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