Journal
JOURNAL OF ALZHEIMERS DISEASE
Volume 28, Issue 4, Pages 735-758Publisher
IOS PRESS
DOI: 10.3233/JAD-2011-111421
Keywords
ADDL; Alzheimer's disease; amyloid-beta; fibrils; immunoassay; microscopy; oligomers; separation techniques; spectroscopy; spectrum analysis; structural analysis
Categories
Funding
- CTMM, the Center for Translational Molecular Medicine
- LeARN [02N-101]
- Merck/MSD
- Virtual Proteins
- BAC
- Cyclotron BV
- LUMC
- VUmc
- MUMC
- RUNMC
- Philips
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Amyloid-beta protein (A beta) accumulation is one of the major hallmarks of Alzheimer's disease and plays a crucial role in its pathogenesis. A beta aggregates into fibrils, but rather than these end-products of the aggregation process, intermediate species, referred to as oligomers, have been identified as the most neurotoxic A beta aggregates. To characterize the different A beta species and to study the aggregation process, a wide range of techniques has been applied over the past years. These techniques aim to visualize the different A beta species and study their structure, to separate them, and to quantify the aggregated A beta forms by immunology-based methods. In this review, we provide an overview and discussion of the most important techniques used for these aims. Often a combination of techniques will be appropriate to obtain the most optimal information.
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