Phosphorylation of a Tyrosine in the Amyloid-beta Protein Precursor Intracellular Domain Inhibits Fe65 Binding and Signaling

The phosphorylation of Tyr-682 residue in the intracellular domain (AID) of amyloid-beta protein precursor (A beta PP) is significantly enhanced in Alzheimer's disease patients' brain. The role of this phosphotyrosine, however, remains elusive. Here we report that phosphorylation of Tyr-682 inhibits the interactions between A beta PP and Fe65, which is the main regulatory mechanism controlling Fe65 nuclear signaling. Furthermore, we show that tyrosine phosphorylation of A beta PP also inhibits interaction of the two other Fe65 family members, Fe65L1 and Fe65L2. Likewise, docking of Fe65, Fe65L1 and Fe65L2 to APLP1 and APLP2, the two other members of the A beta PP-gene family, is abolished by analogous phosphorylation events. Our results indicate that phosphorylation of the cytoplasmic tail of A beta PP on Tyr-682 represents a second mechanism, alternative to A beta PP processing by secretases, that regulates A beta PP/Fe65 downstream signaling pathways.