Journal
NANOSCALE
Volume 7, Issue 29, Pages 12606-12613Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr02164b
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Funding
- Singapore Ministry of Education [RGT19/13, MOE2013-T3-1-002]
- National Research Foundation Singapore through the Singapore MIT Alliance
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We have developed a new type of surface enhanced Raman scattering (SERS) substrate with thiolated graphene oxide (tGO) nanosheets sandwiched between two layers of closely packed plasmonic nano-particles. The trilayered substrate is built up through alternative loading of interfacially assembled plasmonic nanoparticle arrays and tGO nanosheets, followed by coating the nanoparticle surfaces with poly(ethylene glycol) (PEG). Here tGO plays multifunctional roles as a 2D scaffold to immobilized inter-facially assembled plasmonic nanoparticles, a nanospacer to create SERS-active nanogaps between two layers of nanoparticle arrays, and a molecule harvester to enrich molecules of interest via pi-pi interaction. In particular, the molecule harvesting capability of the tGO nanospacer and the stealth properties of PEG coating on the plasmonic nanoparticles collectively lead to preferential positioning of selective targets such as aromatic molecules and single-stranded DNA at the SERS-active nanogap hotspots. We have demonstrated that an SERS assay based on the PEGylated trilayered substrate, in combination with magnetic separation, allows for sensitive, multiplexed signal-off detection of DNA sequences of bacterial pathogens.
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