Journal
NANOSCALE
Volume 7, Issue 25, Pages 10903-10907Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr01187f
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Funding
- Jiangsu University Start-up Fund Senior Professionals [13JDG005]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu University Graduate Project [13A328]
- National Natural Science Foundation of China [31301919]
- National Basic Research Program of China [2012CB114604]
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In recent years, the detection of proteins by using bare graphene oxide (GO) to quench the fluorescence of fluorescein-labeled aptamers has been reported. However, the proteins can be adsorbed on the surface of bare GO to prevent the sensitivity from further being improved. In order to solve this problem, polyethylene glycol (PEG)-protected GO was used to prevent the proteins using thrombin as an example from nonspecific binding. The detection limit was improved compared to bare GO under the optimized ratio of GO to PEG concentration. The results show that our method is a promising technique for the detection of proteins.
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