4.8 Article

Dextran-coated superparamagnetic nanoparticles as potential cancer drug carriers in vivo

Journal

NANOSCALE
Volume 7, Issue 25, Pages 11155-11162

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr01382h

Keywords

-

Funding

  1. Key National Science and Technology Projects on Key New Drug Creation and Manufacturing [2012ZX09506001001]
  2. 973 National ST Major Project [2011CB503900]
  3. National Science Foundation of Shaanxi Province [2012JM2003, 2012JQ4026]
  4. National Natural Science Foundation of China [21303136, 1170101, 81370235]

Ask authors/readers for more resources

Dextran-coated superparamagnetic iron oxide nanoparticles (DSPIONs) have gained considerable interest, because of their biocompatibility and biosafety in clinics. Doxorubicin (Dox), a widely used chemotherapeutic drug, always has limited applications in clinical therapy due to its serious side effects of dose-limiting irreversible cardiotoxicity and myelo suppression. Herein, DSPIONs were synthesized and developed as magnetic carriers for doxorubicin. The Dox-DSPION conjugates were evaluated in the in vitro test of Dox release, which showed pH-dependence with the highest release percentage of 50.3% at pH 5.0 and the lowest release percentage of 11.8% in a physiological environment. The cytotoxicity of DSPIONs and Dox-DSPIONs evaluated by the MTT assay indicated that DSPIONs had no cytotoxicity and the conjugates had significantly reduced the toxicity (IC50 = 1.36 mu g mL(-1)) compared to free Dox (IC50 = 0.533 mu g mL(-1)). Furthermore, confocal microscopic data of cell uptake suggest that less cytotoxicity of Dox-DSPIONs may be attributed to the cellular internalization of the conjugates and sustainable release of Dox from the formulation in the cytoplasm. More importantly, the results from the rabbit VX2 liver tumor model test under an external magnetic field showed that the conjugates had approximately twice the anti-tumor activity and two and a half times the animal survival rate, respectively, compared to free Dox. Collectively, our data have demonstrated that Dox-DSPIONs have less toxicity with better antitumor effectiveness in in vitro and in vivo applications, suggesting that the conjugates have potential to be developed into chemo-therapeutic formulations.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available