Journal
NANOSCALE
Volume 7, Issue 15, Pages 6836-6842Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr00070j
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Funding
- Natural Science Foundation of China [81201141]
- Fundamental Research Funds for Central Universities of China [N130520001]
- Liaoning Provincial Educational Commission [L2014095]
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Amino-functionalized fluorescent carbon dots have been prepared by hydrothermal treatment of glucosamine with excess pyrophosphate. The produced carbon dots showed stabilized green emission fluorescence at various excitation wavelengths and pH environments. Herein, we demonstrate the surface energy transfer between the amino-functionalized carbon dots and negatively charged hyaluronate stabilized gold nanoparticles. Hyaluronidase can degrade hyaluronate and break down the hyaluronate stabilized gold nanoparticles to inhibit the surface energy transfer. The developed fluorescent carbon dot/gold nanoparticle system can be utilized as a biosensor for sensitive and selective detection of hyaluronidase by two modes which include fluorescence measurements and colorimetric analysis.
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