Journal
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume 133, Issue 4, Pages 1184-+Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2013.10.056
Keywords
Group 2 innate lymphoid cell; PGD(2); chemoattractant receptor-homologous molecule expressed on T(H)2 cells; IL-25; IL-33; innate type 2 immunity; adaptive type 2 immunity
Categories
Funding
- Wellcome Trust
- Medical Research Council
- NIHR Biomedical Research Centre Programme
- Oxford Martin School
- British Medical Association (James Trust)
- Oxfordshire Health Services Research Committee Research Grant
- National Institutes of Health
- MRC [MC_UU_12010/5, MC_U105178805, G116/150, G0701693, MC_U137881017] Funding Source: UKRI
- Medical Research Council [MC_U137881017, MC_U105178805, G1000800h, MC_UU_12010/5, G0701693] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0510-10204] Funding Source: researchfish
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Background: Activation of the group 2 innate lymphoid cell (ILC2) population leads to production of the classical type 2 cytokines, thus promoting type 2 immunity. Chemoattractant receptor-homologous molecule expressed on T(H)2 cells (CRTH2), a receptor for prostaglandin D-2 (PGD(2)), is expressed by human ILC2s. However, the function of CRTH2 in these cells is unclear. Objectives: We sought to determine the role of PGD(2) and CRTH2 in human ILC2s and compare it with that of the established ILC2 activators IL-25 and IL-33. Methods: The effects of PGD2, IL-25, and IL-33 on the cell migration, cytokine production, gene regulation, and receptor expression of ILC2s were measured with chemotaxis, ELISA, Luminex, flow cytometry, quantitative RT-PCR, and QuantiGene assays. The effects of PGD(2) under physiologic conditions were evaluated by using the supernatant from activated mast cells. Results: PGD(2) binding to CRTH2 induced ILC2 migration and production of type 2 cytokines and many other cytokines. ILC2 activation through CRTH2 also upregulated the expression of IL-33 and IL-25 receptor subunits (ST2 and IL-17RA). The effects of PGD(2) on ILC2s could be mimicked by the supernatant from activated human mast cells and inhibited by a CRTH2 antagonist. Conclusions: PGD(2) is an important and potent activator of ILC2s through CRTH2 mediating strong proallergic inflammatory responses. Through IgE-mediated mast cell degranulation, these innate cells can also contribute to adaptive type 2 immunity; thus CRTH2 bridges the innate and adaptive pathways in human ILC2s.
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