Journal
NANOSCALE
Volume 7, Issue 32, Pages 13511-13520Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr01851j
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Funding
- Swiss National Science Foundation [145756]
- Imperial College London
- Intensive Care Foundation
- National Institute for Health Research (NIHR) through the Comprehensive Biomedical Research Centre based at Imperial College Healthcare NHS Trust and Imperial College London
- NIHR
- EPSRC [EP/K020641/1]
- Engineering and Physical Sciences Research Council [EP/K020641/1] Funding Source: researchfish
- National Institute for Health Research [NIHR/CS/009/007] Funding Source: researchfish
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Sepsis is a severe medical condition and a leading cause of hospital mortality. Prompt diagnosis and early treatment has a significant, positive impact on patient outcome. However, sepsis is not always easy to diagnose, especially in critically ill patients. Here, we present a conceptionally new approach for the rapid diagnostic differentiation of sepsis from non-septic intensive care unit patients. Using advanced microscopy and spectroscopy techniques, we measure infection-specific changes in the activity of nano-sized cell-derived microvesicles to bind bacteria. We report on the use of a point-of-care-compatible microfluidic chip to measure microvesicle-bacteria aggregation and demonstrate rapid (<= 1.5 hour) and reliable diagnostic differentiation of bacterial infection from non-infectious inflammation in a double-blind pilot study. Our study demonstrates the potential of microvesicle activities for sepsis diagnosis and introduces microvesiclebacteria aggregation as a potentially useful parameter for making early clinical management decisions.
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