Mast cell TNF receptors regulate responses to Mycoplasma pneumoniae in surfactant protein A (SP-A)-/- mice

Background: Mycoplasma pneumoniae (Mp) frequently colonizes the airways of patients with chronic asthma and likely contributes to asthma exacerbations. We previously reported that mice lacking surfactant protein A (SP-A) have increased airway hyperresponsiveness (AHR) during M pneumoniae infection versus wild-type mice mediated by TNF-alpha. Mast cells (MCs) have been implicated in AHR in asthma models and produce and respond to TNF-alpha. Objective: Determine the contribution of MC/TNF interactions to AHR in airways lacking functional SP-A during Mp infection. Methods: Bronchoalveolar lavage fluid was collected from healthy and asthmatic subjects to examine TNF-alpha levels and M pneumoniae positivity. To determine how SP-A interactions with MCs regulate airway homeostasis, we generated mice lacking both SP-A and MCs (SP-A(-)/(-)Kit(W-sh/W-sh)) and infected them with M pneumoniae. Results: Our findings indicate that high TNF-alpha levels correlate with M pneumoniae positivity in human asthmatic patients and that human SP-A inhibits M pneumoniae-stimulated transcription and release of TNF-alpha by MCs, implicating a protective role for SP-A. MC numbers increase in M pneumoniae-infected lungs, and airway reactivity is dramatically attenuated when MCs are absent. Using SP-A(-/-)Kit(W-sh/W-sh) mice engrafted with TNF-alpha(-/-) or TNF receptor (TNF-R)(-/-) MCs, we found that TNF-alpha activation of MCs through the TNF-R, but not MC-derived TNF-alpha, leads to augmented AHR during M pneumoniae infection when SP-A is absent. Additionally, M pneumoniae-infected SP-A(-/-)Kit(W-sh/W-sh) mice engrafted with TNF-alpha(-/-) or TNF-R-/- MCs have decreased mucus production compared with that seen in mice engrafted with wild-type MCs, whereas burden was unaffected. Conclusion: Our data highlight a previously unappreciated but vital role for MCs as secondary responders to TNF-alpha during the host response to pathogen infection. (J Allergy Clin Immunol 2012; 130:205-14.)