Journal
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume 129, Issue 6, Pages 1621-+Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2012.01.052
Keywords
Asthma; airway; macrophage; chemokines; inflammation; IL-13; IFN-gamma; CD11b; dendritic cell; macrophage-derived chemokine; thymus and activation-regulated chemokine
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Funding
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [AI076315, AI083534]
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Background: IL-13 in the airway induces pathologies that are highly characteristic of asthma, including mucus metaplasia, airway hyperreactivity (AHR), and airway inflammation. As such, it is important to identify the IL-13-responding cell types that mediate each of the above pathologies. For example, IL-13' s effects on epithelium contribute to mucus metaplasia and AHR. IL-13' s effects on smooth muscle also contribute to AHR. However, it has been difficult to identify the cell types that mediate IL-13-induced airway inflammation. Objective: We sought to determine which cell types mediate IL-13-induced airway inflammation. Methods: We treated the airways of mice with IL-13 alone or in combination with IFN-gamma. We associated the inhibitory effect of IFN-gamma on IL-13-induced airway inflammation and chemokine production with cell types in the lung that coexpress IL-13 and IFN-gamma receptors. We then evaluated IL-13-induced responses in CD11c promoter-directed diphtheria toxin receptor-expressing mice that were depleted of both dendritic cells and alveolar macrophages and in CD11b promoter-directed diphtheria toxin receptor-expressing mice that were depleted of dendritic cells. Results: Dendritic cell and alveolar macrophage depletion protected mice from IL-13-induced airway inflammation and CCL11, CCL24, CCL22, and CCL17 chemokine production. Preferential depletion of dendritic cells protected mice from IL-13-induced airway inflammation and CCL22 and CCL17 chemokine production but not from IL-13-induced CCL11 and CCL24 chemokine production. In either case mice were not protected from IL-13-induced AHR and mucus metaplasia. Conclusions: Pulmonary dendritic cellsand alveolarmacrophages mediate IL-13-induced airway inflammation and chemokine production. (J Allergy Clin Immunol 2012; 129: 1621-7.)
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