Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 11, Issue 4, Pages 835-843Publisher
ELSEVIER
DOI: 10.1016/j.nano.2015.01.012
Keywords
Gene therapy; Transfection; Non-viral vectors; In vivo; Brain; Optogenetics
Funding
- Spanish Government [MAT2012-39290-C02-01]
- Research Chair in Retinosis Pigmentaria Bidons Egara
- National Organization of the Spanish Blind (ONCE)
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This work demonstrates the successful long-term transfection in vivo of a DNA plasmid vector in rat visual cortex neurons using the magnetofection technique. The transfection rates reached values of up to 97% of the neurons after 30 days, comparable to those achieved by viral vectors. Immunohistochemical treatment with anti-EGFP antibodies enhanced the detection of the EYFP-channelrhodopsin expression throughout the dendritic trees and cell bodies. These results show that magnetic nanoparticles offer highly efficient and enduring in vivo high-rate transfection in identified neurons of an adult mammalian brain and suggest that the magnetotechnique facilitates the introduction of large functional genetic material like channelrhodopsin with safe non-viral vectors using minimally invasive approaches. (C) 2015 Elsevier Inc. All rights reserved.
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