Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection

Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective turn on plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of similar to 0.1 nM (200 amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination. (C) 2015 Elsevier Inc. All rights reserved.