Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 11, Issue 6, Pages 1585-1596Publisher
ELSEVIER
DOI: 10.1016/j.nano.2015.04.010
Keywords
Nanoparticle; Intracellular trafficking; Acidification; pH responsiveness; Rab-family
Funding
- Deutsche Forschungsgemeinschaft (DFG) [SPP 1313, LA1013/13-1, MA 3271/3-1, SFB1066]
- Max Planck Graduate Center with the Johannes Gutenberg University Mainz (MPGC)
Ask authors/readers for more resources
Many nanoparticular drug delivery approaches rely on a detailed knowledge of the acidification process during intracellular trafficking of endocytosed nanoparticles (NPs). Therefore we produced a nanoparticular pH sensor composed of the fluorescent pH-sensitive dual wavelength dye carboxy seminaphthorhodafluor-1 (carboxy SNARF-1) coupled to the surface of amino-functionalized polystyrene NPs (SNARF-1-NP). By applying a calibration fit function to confocal laser scanning microscopy (CLSM) images, local pH values were determined. The acidification and ripening process of endo/lysosomal compartments containing nanoparticles was followed over time and was found to progress up to 6 h to reach an equilibrium pH distribution (maximum pH 5.2 [+/- 0.2]). The SNARF-1-NP localization in endo/lysosomal compartments was confirmed by transmission electron microscopy (TEM) and quantitative co-localization analysis with fluorescent endolysosomal marker Rab-proteins by confocal laser scanning microscopy (CLSM). The herein described nanoparticular pH-sensor is a versatile tool to monitor dynamic pH processes inside the endolysosomal compartments. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available