Journal
NANOMEDICINE
Volume 10, Issue 7, Pages 1063-1076Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.14.160
Keywords
cell imaging; cellular therapy; drug delivery; endocytosis; leukemia; nanoparticles; NP release; T lymphocytes; tumor
Funding
- Jurgen Knop-Program of the Forschungszentrum Immunologie at Mainz University
- Naturwissenschaftlich-Medizinisches Forschungszentrum (NMFZ)
- Deutsche Forschungsgemeinschaft (DFG) [KFO183-TP5]
- DFG [SPP1313, MA 3271/3-1, LA1013/13-1, SFB1066]
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Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells. Materials & methods: Uptake, release and toxicity of various polymeric NPpreparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-gamma-ELISPOT and 51Chromium-release assays. Results: Aminofunctionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h. Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo.
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