Journal
NANO LETTERS
Volume 15, Issue 5, Pages 3134-3138Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b00189
Keywords
DNA nanotechhology; artificial ion channels; DNA-tiles; single-molecule; lipid bilayer; self-assembly
Categories
Funding
- Winton Programme for the Physics of Sustainability
- Gates Cambridge
- Oppenheimer Ph.D. studentship
- ERASMUS programme
- Herchel Smith postdoctoral fellowship
- DFG Nanosystems Initiative Munich
- ERC [261101]
- Oxford Nanopore Technologies
- European Research Council (ERC) [261101] Funding Source: European Research Council (ERC)
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Self-assembled DNA nanostructures have been used to create man-made transmembrane channels in lipid bilayers. Here, we present a DNA-tile structure with a nominal subnanometer channel and cholesterol-tags for membrane anchoring. With an outer diameter of 5 nm and a molecular weight of 45 kDa, the dimensions of our synthetic nanostructure are comparable to biological ion channels. Because of its simple design, the structure self-assembles within a minute, making its creation scalable for applications in biology. Ionic current recordings demonstrate that the tile structures enable ion conduction through lipid bilayers and show gating and voltage-switching behavior. By demonstrating the design of DNA-based membrane channels with openings much smaller than that of the archetypical six-helix bundle, our work showcases their versatility inspired by the rich diversity of natural membrane components.
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