Journal
NANO LETTERS
Volume 16, Issue 1, Pages 138-144Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b03331
Keywords
nanopores; tRNA; single molecule; RNA; SVM
Categories
Funding
- National Institutes of Health [HG006873, HG 006323, GM080376]
Ask authors/readers for more resources
It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through similar to 3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available