Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 38, Pages 9914-9922Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b03098
Keywords
eugenol; cadmium; hydrogen sulfide; glutathione; Brassica rapa
Funding
- National Key Research and Development Program of China [2017YFD0201105]
- National Natural Science Foundation of China [31771705]
- Program for Science and Technology Innovation Team in Universities of Henan Province [16IRTSTHN007]
- Jiangsu Agricultural Science Innovative Foundation [CX(14)5063]
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Eugenol, a plant-derived small compound, shows great medicinal potential. However, whether and how eugenol regulates crop physiology remains elusive. Here we reported that eugenol induced Cd (cadmium) tolerance in the root of Brassica rapa. Roots were treated with eugenol and CdCl2 simultaneously (eugenol + Cd) or pretreated with eugenol followed by CdCl2 treatment (eugenol -> Cd). Eugenol significantly attenuated Cd-induced growth inhibition, ROS accumulation, oxidative injury, and cell death, which were confirmed by in vivo histochemical analysis. Eugenol remarkably decreased free Cd2+ accumulation in root. Eugenol intensified GSH (glutathione) accumulation in roots upon CdCl2 exposure, which explained the decrease in free Cd2+ and attenuation of oxidative injury. Eugenol stimulated endogenous H2S (hydrogen sulfide) generation by upregulating the expression of BrLCD (L-cysteine desulfhydrase) and BrDCD (D-cysteine desulfhydrase) as well as their enzymatic activities in CdCl2-treated root. Application of H2S biosynthesis inhibitor or H2S scavenger led to the decrease in endogenous H2S level in Cd-treated root, which further compromised all the above effects of eugenol. These findings suggested that eugenol triggered H2S -> GSH signaling cassette in plants to combat Cd stress, which shed new light on eugenol-modulated plant physiology and the interaction between eugenol and H2S.
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