Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 40, Pages 9927-9935Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf502239d
Keywords
mixed microbes; cane molasses wastewater; 2-phenyethanol; comparative metabolomics; metabolic mechanism
Funding
- National 973 Project of China [2013CB733600]
- National Natural Science Foundation of China [21236005]
- Program of Introducing Talents of Discipline to Universities [B06006]
Ask authors/readers for more resources
The mixed microbes coculture method in cane molasses wastewater (CMW) was adopted to produce 2-phenylethanol (2-PE). Comparative metabolomics combined with multivariate statistical analysis was performed to profile the differences of overall intracellular metabolites concentration for the mixed microbes cocultured under two different fermentation conditions with low and high 2-PE production. In total 102 intracellular metabolites were identified, and 17 of them involved in six pathways were responsible for 2-PE biosynthesis. After further analysis of metabolites and verification by feeding experiment, an overall metabolic mechanism hypothesis for the microbial mixed cultures (MMC) utilizing CMW for higher 2-PE production was presented. The results demonstrated that the branches of intracellular pyruvate metabolic flux, as well as the flux of phenylalanine, tyrosine, tryptophan, glutamate, proline, leucine, threonine, and oleic acid, were closely related to 2-PE production and cell growth, which provided theoretical guidance for domestication and selection of species as well as medium optimization for MMC metabolizing CMW to enhance 2-PE yield.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available