Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 1, Pages 75-84Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf5046359
Keywords
dimethyl phthalate; trypsin; interaction; spectroscopy; molecular docking; atomic force microscopy
Funding
- National Natural Science Foundation of China [31460422, 21167013]
- Natural Science Foundation of Jiangxi Province [20143ACB20006, 20142BAB204001]
- Joint Specialized Research Fund for the Doctoral Program of Higher Education [20123601110005]
- Program of Jiangxi Provincial Department of Science and Technology [20141BBG70092]
- Research Program of State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZB-201305, SKLF-ZZA-201302, SKLF-KF-201203]
Ask authors/readers for more resources
Dimethyl phthalate (DMP) is widely used as a plasticizer in industrial processes and has been reported to possess potential toxicity to the human body. In this study, the interaction between DMP and trypsin in vitro was investigated. The results of fluorescence, UV-vis, circular dichroism, and Fourier transform infrared spectra along with cyclic voltammetric measurements indicated that the remarkable fluorescence quenching and conformational changes of trypsin resulted from the formation of a DMP-trypsin complex, which was driven mainly by hydrophobic interactions. The molecular docking and trypsin activity assay showed that DMP primarily interacted with the catalytic triad of trypsin and led to the inhibition of trypsin activity. The dimensions of the individual trypsin molecules were found to become larger after binding with DMP by atomic force microscopy imaging. This study offers a comprehensive picture of DMP-trypsin interaction, which is expected to provide insights into the toxicological effect of DMP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available