Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 31, Pages 7698-7706Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf5019752
Keywords
bioavailability; gut microbial metabolism; colon; polyphenols; proanthocyanidins; flavanols; HPLC-ESI-MS/MS
Funding
- European Union [244995]
- Universitat Rovira i Virgili-Banco Santander [2011 LINE-13, 2011 LINE-12]
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This study describes the development and validation of a liquid chromatography mass spectrometry method for determination of a large number of flavanol colonic derivatives in biological samples. The method was validated with rat plasma after the intake of grape seed flavanols. The minimum plasma volume necessary to maintain good recovery values within the range of 83-110% for all of the standards was determined by micro solid-phase extraction (mu-SPE). In total, 16 commercial standards were used to measure 30 different phenolic compounds present at low concentration levels (micromolar). The chromatographic method enabled reliable quantification of plasma colonic flavanol derivatives with low limits of detection and quantification, achieving values of 0.03 nM and 0.10 nM, respectively. The developed method can be readily applied to determine all of the flavanol metabolites that are most likely responsible for the majority of biological effects of poorly absorbed flavanols.
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