Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 25, Pages 5827-5834Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf500950p
Keywords
Co3O4 nanoparticles; oxidase mimic; colorimetry; sulfite; mechanism of oxidase-like activity
Funding
- National Natural Science Foundation of China [21375053]
- Specialized Research Fund for the Doctoral Program of Higher Education [20130211110039]
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In this paper, we first discovered that Co3O4 nanoparticles (NPs) possess intrinsic oxidase-like activity and can catalytically oxidize peroxidase substrates, such as 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 3,3',5,5'-tetramethylbenzidine (TMB), to form colored products, in the absence of exogenously added H2O2. The presence of sulfite inhibited the TMB-O-2-Co3O4 NPs reaction system and caused a change in color of the reaction system. On the basis of this phenomenon, a colormetric approach to detect sulfite was established with a good linear relationship ranging from 0.2 X 10(-6) to 1.6 X 10(-5) M and a detection limit of 5.3 x 10(-8) M. The method was used to detect sulfite in foods. Good recoveries ranging from 93.8% to 100.5% were obtained. Furthermore, the mechanism was studied and results showed that the oxidase-like activity of the Co3O4 NPs was not from center dot OH or O-2(center dot-). radical generated. It may probably originate from their ability to transfer an electron between the peroxidase substrate and oxygen absorbed on the surface of the Co3O4 NPs.
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