Dispersive Micro-Solid-Phase Extraction Using Mesoporous Hybrid Materials for Simultaneous Determination of Semivolatile Compounds from Plant Tea by Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

This report described the use of mesoporous hybrid materials (MHM) in a dispersive micro-solid phase extraction procedure to extract semivolatile compounds from plant tea that were then analyzed by ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Dihydrotanshinone. I, tanshinone I, cryptotanshinone, and tanshinone IIA were selected as the model compounds, and the extraction parameters, including mesoporous concentration, extraction time, sample agitation and desorption solvents, were optimized. The interaction with the analytes and large surface area of the MHM facilitated the adsorption of analytes. The method showed good linearity, with correlation coefficients >0.9980 in the ragne 0.25-100 ng/mL, and low limits of detection (0.012-0.046 pg). Finally the recovery values were 91-103% for Danshen tea, 89-102% for Danshen, and 88-96% for tanshinone capsules. The results showed that the proposed method was suitable for the extraction and determination of tanshinones in complex samples.