Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 13, Pages 2935-2945Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf404826u
Keywords
Brassica napus L. var. napus; flavonoids; hydroxycinnamic acid; HPLC-PDA-ESI(-)-MSn/HRMS
Funding
- NSFC projects [31330057, 31171581]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- SRFDP project [20123250110009]
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A comprehensive description of flavonoids and hydroxycinnamic acid derivatives in Brassica napus L. var. napus seeds is important to improve rapeseed quality. HPLC-PDA-ESI(-)-MSn/HRMS has been broadly applied to study phenolic compounds in plants. In the present study, crude phenolic compounds extracted from rapeseed were subjected to column chromatography, alkaline hydrolysis, and HPLC-PDA ESI(-)-MSn/HRMS analysis. A total of 91 flavonoids and hydroxycinnamic acid derivatives were detected, including 39 kaempferol derivatives, 11 isorhamnetin derivatives, 5 quercetin derivatives, 6 flavanols and their oligomers, and 30 hydroxycinnamic acid derivatives. A total of 78 of these compounds were tentatively identified; of these, 55 were reported for the first time in B. napus L. var. napus and 24 were detected for the first time in the genus Brassica. This research enriches our knowledge of the phenolic composition of rapeseed and provides a reliable guide for the selection of rapeseed with valuable breeding potential.
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