Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 39, Pages 9420-9427Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf5022785
Keywords
boar taint; indole; skatole (3-methylindole); androstenone (s alpha-androst-16-en-3-one); GC-MS; dynamic headspace; trap/purge; discrimination; at-line
Funding
- Danish National Advanced Foundation
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This work outlines an optimized gas chromatography-mass spectrometry (GC-MS) based protocol for screening of the presence of the three boar-taint-producing compounds indole, skatole (3-methylindole), and androstenone (5 alpha-androst-16-en-3-one) in porcine fat. The study shows that an accuracy suitable for sample screening can be achieved even when spped and ease of sample handling is prioritized and without the use of internal standards. The method provides levels of detection of 82 ng/g for indole, 97 ng/g for skatole and 623 ng/g for androstenone (in a 2 g natural backfat matrix). A least-squares approach of predicting the sample analyte mass from instrument response is shown to have prediction errors (root-mean-square error) of 96 ng/g for indole, 94 ng/g for skatole, and 331.3 ng/g for androstenone. The method is intended for discrimination of samples in risk groups and a scheme is proposed to turn the GC-MS response of the boar-taint analytes into risk classes.
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