Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 26, Pages 6190-6198Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf501414h
Keywords
Olea europaea; drying; in vitro digestion; antioxidant potential; byproduct
Funding
- Generalitat Valenciana [PROMETEO/2010/062, PROMETEO/2012/007, ACOMP/2013/93]
- Ministerio de Educacion, Cultura y Deporte of Spain
- Catedra AINIA (Universitat Politecnica de Valencia)
- Ministerio de Ciencia e Innovacion [AGL2011-29857-C03-03]
- CIBERobn [CB12/03/30038]
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Olive leaves are rich in bioactive compounds, which are beneficial for humans. The objective of this work was to assess the influence of processing conditions (drying and extraction) of olive leaves on the extract's bioaccessibility. Thus, extracts obtained from dried olive leaves (hot air drying at 70 and 120 degrees C or freeze-drying) by means of conventional or ultrasound-assisted extraction were subjected to in vitro digestion. Antioxidant capacity, total phenolic content, and HPLC-DAD/MS/MS analysis were carried out during digestion. The dehydration treatment used for the olive leaves did not have a meaningful influence on bioaccessibility. The digestion process significantly (p < 0.05) affected the composition of the extracts. Oleuropein and verbascoside were quite resistant to gastric digestion but were largely degraded in the intestinal phase. Nevertheless, luteolin-7-0-glucoside was the most stable polyphenol during the in vitro simulation (43% bioaccessibility). Therefore, this compound may be taken into consideration in further studies that focus on the bioactivity of olive leaf extracts.
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