Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 32, Pages 8068-8074Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf5019356
Keywords
Crocus sativus L.; aqueous extract; trans-4-GG; trans-3-Gg picrocrocin; safranal; HPLC-DAD
Ask authors/readers for more resources
The aim of this work was to propose a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for determining the three main compounds responsible for determining the quality of saffron (crocetin esters, picrocrocin, and safranal) by preparing an aqueous extract according to the ISO 3632 standard to solve the difficulty that this standard has for aroma and taste determination by ultraviolet visible spectroscopy. Toward this aim, laboratory-isolated picrocrocin, a safranal standard with a purity of >= 88%, trans-crocetin di(beta-D-gentiobiosyl) ester (trans-4-GG) and trans-crocetin (beta-D-glucosyl)-(beta-D-gentiobiosyl) ester (trans-3-Gg) standards, both with a purity of >= 99%, and 50 different saffron spice samples from Italy, Iran, Greece, and Spain were used in the intralaboratory validation of the HPLC method. The analytical method proposed was adequate in terms of linearity, selectivity, sensitivity, and accuracy for determining the three foremost parameters that define the quality of saffron using only a saffron solution prepared according to the ISO 3632 standard.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available