UHPLC-PDA-ESUHRMSn Profiling Method To Identify and Quantify Oligomeric Proanthocyanidins in Plant Products

oligomeric proanthocyanidins were successfully identified by UHPLC-PDA-HRMS in a selection of plant derived materials (jujube fruit, Fuji apple, fruit pericarps of litchi and mangosteen, dark chocolate, and grape seed and cranberry extracts). The identities of 247 proanthocyanidins were theoretically predicted by computing high-accuracy masses based on the degree of polymerization flavan-3-ol components and the number of A type bonds identification of doubly or triply charged ions of 50 PAs was made on the basis of theoretical calculations. A single catechin standard and molar relative response factors (MRRFs) were used to quantify the well-seperated PAs. The ration of the SIM peak counts were used to quantify each of the unseperated isomers. This is the first report of direct determination of each of the proanthocyanidins in plant derived foods and proanthocyanidins containing an epifisetinidol unit in grape seeds.