Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 15, Pages 3405-3409Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf5004539
Keywords
benzo[e]indolium; bisulfite sensing; chemosensor; ratiometric fluorescence
Funding
- National Natural Science Foundation of China [21301126]
- Shanxi Province Science Foundation for Youths [2013021009-3]
- Youth Foundation of Taiyuan University of Technology [2012L018, 2012L062]
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A ratiometric fluorescent probe based on a positively charged benzo[e]indolium moiety for bisulfite is reported. The bisulfite underwent a 1,4-addition reaction with the C-4 atom in the ethylene group. This reaction resulted in a large emission wavelength shift (Delta lambda = 106 nm) and an observable fluorescent color change from orange to cyan. The reaction could be completed in 90 s in a PBS buffer solution and displayed high selectivity and sensitivity for bisulfite. A simple paper test strip system was developed to detect bisulfite rapidly. Probe 1 was used to detect bisulfite in real samples with good recovery.
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