Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 46, Pages 10911-10918Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf402483c
Keywords
turmeric; curcumin; demethoxycurcumin; bisdemethoxycurcumin; method validation; high-performance liquid chromatography (HPLC)
Funding
- Dankook University
Ask authors/readers for more resources
Turmeric is a plant in the Zingiberaceae family which contains curcuminoids as anticancer agents and has been widely used as a main ingredient in curry powder. However, there is a lack of suitable high performance liquid chromatography-diode array detection (HPLC-DAD) methods for reducing sample preparation time and peak resolution improvement of curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin). No significant differences in yield concentrations were observed after 60 min of heat-refluxed extraction (p < 0.05). Simultaneous chromatographic separation of all three curcuminoids achieved satisfactory results with a separation factor of 1.08 and a resolution factor of 3.39 with validation results in compliance with FDA guidelines. The expanded relative measurement uncertainty results 5.71-6.60 complied with CODEX draft. The method was successfully applied to the turmeric samples (n = 107, range 2.70-4.41 g/100 g, total curcuminoids 3.58 g/100 g). These results show that heat-refluxed extraction can be carried out easily with excellent precision and accuracy of total curcuminoids in turmeric samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available