Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 11, Pages 2755-2761Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf304768p
Keywords
dihydroquercetin; antioxidant activity; Nrf2; antioxidant genes
Funding
- Special Fund of Forestry Industrial Research for Public Welfare of China [201004040]
- Program for Importation of International Advanced Forestry Science and Technology
- National Forestry Bureau [2012-4-06]
- Heilongjiang Province Science Foundation for Excellent Youths [JC200704]
- Project for Distinguished Teacher Abroad, Chinese Ministry of Education [MS2010DBLY031]
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Dihydroquercetin (DHQ) is a well-known antioxidant agent. In the present investigation, we reported for the first time that DHQ stimulates the expression of phase II detoxifying enzymes through the Nrf2-dependent signaling pathway. The IC50 values of DHQ for reduction of 2,2-diphenyl-1-picrylhydrazol (DPPH), reducing power assay, lipid peroxidation assay, and xanthine oxidase inhibition were 5.96, 4.31, 2.03, and 13.24 mu M, respectively. DHQ possessed considerable protective activity from oxidative DNA damage. A luciferase reporter assay also demonstrated that DHQ-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to the ARE (antioxidant response element) enhancer sequence. Furthermore, Western blotting and luciferase assay revealed DHQ activated ERK1/2, Akt, and JNK signaling pathways, subsequently leading to Nrf2 nuclear translocation. DHQ upregulated the Nrf2-related antioxidant genes heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase-1 (NQO1), and glutamate-cysteine ligase modifier subunits. Inhibition of Nrf2 by siRNA reduced DHQ:induced upregulation of these antioxidant genes.
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