Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 26, Pages 6288-6292Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf401104g
Keywords
jasmonic acids; chiral separation; gas chromatography/mass spectrometry; monolithic material sorptive extraction; endogenous jasmonates
Funding
- National Scientific Foundation of China [90817103, 30973672]
- Doctoral Fund of Ministry of Education of China [20110141110024]
- Hubei Provincial Scientific Foundation [2011CDB475]
- Fundamental Research Funds for the Central Universities
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A GC/MS method with monolithic material sorptive extraction (MMSE) pretreatment was developed to determine contents of the enantiomers of jasmonic acid and methyl jasmonate in flowers. To optimize MMSE extraction, several MMSE parameters were investigated, including extraction temperature, extraction time, and extraction solvent. Under the optimal conditions, extraction efficiency was good. Using the selected-ion monitoring mode, the limit of detection (LOD, S/N = 3) for methyl jasmonates was 0.257 ng/mL. The limit of quantitation (LOQ SIN = 10) was 0.856 ng/mL. The linearity range was 1-100 ng/mL. The average recovery of methyl jasmonate at lower concentration was 116.8% (2 ng/mL). The relative standard deviation of methyl jasmonate contents determined within the linear range of detection was less than or equal to 15% of the mean determined level. The proposed method is rapid, sensitive, and competently applied to the determination of jasmonic acid and methyl jasmonate enantiomers in flowers.
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