Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 3, Pages 561-568Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf304470k
Keywords
Methoxyphenolic compounds; Plackett-Burman design; Box-Behnken design; pu-erh tea; solid-phase microextraction; gas chromatography-mass spectrometry
Funding
- Cheung Kong Scholars and Innovative Research Team Program in University [IRT1166]
- 863 (Hi-Tech Research and Development Program of China) Program [SS2012AA023408]
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A method based on headspace solid-phase microextraction coupled with gas chromatography mass spectrometry was developed for the analysis of volatile methoxyphenolic compounds in pu-erh tea. Six fibers with different polarities were initially evaluated. The 75 mu m carboxen/polydimethylsiloxane fiber exhibited the highest extraction efficiency and was selected for further optimization. A Plackett-Burman design was used to screen for the brewing proportion of tea and water, amount of pu-erh tea, ionic strength, extraction time, extraction temperature, desorption time, rate of agitation, and equilibrium time. A Box-Behnken design was then applied to optimize the significant factors. Under optimal conditions, the proposed method affords a wide range of linearity, high linear regression coefficients (0.996-0.999), less than 9.0% repeatability of relative standard deviation, and limits of detection ranging from 2.31 to 21.80 ng/g. The proposed method has satisfactory accuracy, with recoveries of 79.08-113.9%. This method was successfully applied for the analysis of pu-erh tea samples.
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