Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 8, Pages 1810-1817Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf304034e
Keywords
melamine; subnanomolar; electrochemical; colorimetric
Funding
- NSFC [21075107, 21275124, 21275125]
- Foundation of Jiangsu Educational Committee [12KJB150022]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Natural Science Foundation of Jiangsu Province [BK2012247]
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Based on the synergetic effect of the electrochemical accumulation process and the signal amplification of enzymes, a new sensitive method has been developed for the detection of subnanomolar melamine. There are two steps involved in the sensor construction process: (1) accumulation of melamine on an electrode by cyclic voltammetric method and (2) chemical coupling of horseradish peroxidase (HRP) with the accumulated melamine through the linkage of glutaraldehyde. The coupled HRP catalyzes the oxidation of guaiacol to generate an amber-colored product. Quantitative analysis of melamine is performed by measuring the absorption intensities of the colored product. Under the optimal conditions, the method showed a wide linearity in the concentration range from 1.0 x 10(-11) to 1.0 x 10(-8) M for melamine detection. Moreover, it has been successfully applied to detect melamine in different infant formula powders and fish feed samples.
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