Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 60, Issue 38, Pages 9673-9679Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf301827y
Keywords
Geotrichum sp lipase; Pichia pastoris; surface display; whole-cell biocatalyst; polyunsaturated fatty acid
Funding
- National Natural Science Foundation of People's Republic of China (NSFC) [31070089, 31170078]
- National High Technology Research Development Program of People's Republic of China (863 Program) [2011AA02A204]
- Innovation Foundation of HUST [2011TS100]
Ask authors/readers for more resources
Geotrichum sp. lipase (GSL) was first displayed on the cell wall of Pichia pastoris on the basis of the a-agglutinin anchor system developed in Saccharomyces cerevisiae. Surface display levels were monitored using Western blotting, immunofluorescence miscroscopy, and fluorescence-activated cell sorting analysis. Lipase activity of the yeast whole cells reached a maximum at 273 +/- 2.4 U/g of dry cells toward olive oil after 96 h of culture at 30 degrees C, with optimal pH and temperature at 7.5 and 45 degrees C, respectively. Displayed GSL exhibited relatively high stability between pH 6.0 and 8.0 and retained >70% of the maximum activity. The surface-displayed lipase retained 80% of its original activity after incubation at 45 degrees C for 4 h. Moreover, the GSL-displaying yeast whole cells were then used as a biocatalyst to enrich eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil on the basis of selective hydrolysis. As a result, EPA and DHA increased from 1.53 and 24.1% in the original fish oil to 1.85 and 30.86%, which were increases of 1.21- and 1.29-fold, respectively. The total yield of EPA and DHA reached 46.62%.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available