Simultaneous Determination of Four Biogenic and Three Volatile Amines in Anchovy by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry

A new method has been developed and validated for the simultaneous determination of four biogenic (putrescine, cadaverine, histamine, and tyramine) and three volatile amines (trimethylamine, triethylamine, and tripropylamine) in anchovy. Separation and determination of the selected compounds were carried out by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using an electrospray ionization source (ESI) in positive ion mode. Chromatographic separation was carried out using an aqueous solution of formic acid (0.1%) and methanol as mobile phase in gradient mode. The method was validated, and mean recoveries were evaluated at three concentration levels (75, 150, and 250 mu g/kg), ranging from 70 to 110% at the three levels assayed. Intra- and interday precision, expressed as relative standard deviation (RSD), were lower than 15% and 20%, respectively. Limits of quantitation (LOQs) were 25 mu g/kg for all cases, except for that of TMA, which was set at 60 mu g/kg. The developed procedure was applied to determine the target compounds in anchovy samples stored during 7 days at 4 degrees C, observing the increasing in the concentration of these compounds at longer storage time.