Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 60, Issue 35, Pages 8590-8599Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf3018832
Keywords
Anoectochilus formosanus; calcium absorption; type II arabinogalactan; prebiotic
Funding
- National Sciences Council of ROC [NSC-96-2317-B-039-003]
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Anoectochilus formosanus (Orchidaceae) is a folk medicine in Asia. This study investigated the in vivo and in vitro prebiotic effects of an aqueous extract of A. formosanus (SAEAF) and of an indigestible polysaccharide (AFP) isolated from SAEAF. Chemical analyses showed AFP was mainly composed of arabinogalactan type II (AG-II), with an average molecular weight of 29 kDa. Following 4 weeks of oral administration to rats, SAEAF exhibited prebiotic effects including a decrease in cecum pH and increases of calcium absorption and fecal bifidobacteria. Furthermore, through a bioactivity-guided separation strategy, AFP was proven to be a bifidogenic component in vitro fecal strains fermentation and in vivo administration to mice. In RT-PCR analysis of Bifidobacterium, AFP increased the expression of ABC transporter related to nutrient uptake. Thus, AFP, a polysaccharide from A. formosanus, was demonstrated to be a prebiotic that has a positive health effect on gut microbiota.
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