Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 60, Issue 3, Pages 791-798Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf204233u
Keywords
Tunisian olive leaves; phenolic compounds; microwave-assisted extraction; high-performance liquid chromatography; time-of-flight mass spectrometry; tandem mass spectrometry
Funding
- Tunisian Ministry of Higher Education and Scientific Research
- Spanish Ministry of Education and Science [AGL2011-29857-C03-02]
- Andalusian Regional Government Council of Innovation and Science [P09-CTS-4564, P10-FQM-6563, P11-CTS-7625]
- University of Granada for the GREIB [GREIB.PYR-2011-02]
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In the present work, a simple and rapid method for the extraction of phenolic compounds from olive leaves, using micro extract (MAE) technique, has been developed. The experimental variables that affect the MAE process, such as the solvent, type and composition, microwave temperature, and extraction time, were optimized using a univariate method. The obtained extracts were analyzed by using high-performance liquid chromatography (HPLC) coupled to electrospray time-of-flight mass spectrometry (ESI-TOF-MS) and electrospray ion trap tandem mass spectrometry (ESI-IT-MS2) to prove the MAE extraction efficiency. The optimal MAE conditions were methanol:water (80:20, v/v) as extracting solvent, at a temperature egued, to 80 degrees C for 6 man. Under these conditions, several phenolic compounds could be characterized by HPLC-ESI-MS/M. A compared to the conventional method, MAE can be used as an alternative extraction method for the characterization of phenolic compounds from olive leaves due to its efficiency and speed.
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