Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 23, Pages 12368-12374Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf203339t
Keywords
chicken egg; anti-protease; expression; sexual maturation; antibacterial activity
Funding
- French National Research Agency (OVO-mining) [ANR-09-BLAN-0136]
- European Community (RESCAPE) [FOOD CT 2006-03018]
- Region Centre
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0136] Funding Source: Agence Nationale de la Recherche (ANR)
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Chicken egg ovoinhibitor is a multidomain Kazal-type serine protease inhibitor with unknown function. Comparison of expression between different tissues indicated that ovoinhibitor is highly expressed in the magnum and liver followed by the uterus, which secrete egg white, egg yolk, and eggshell precursors, respectively. The results also revealed that ovoinhibitor expression is increased in the liver during sexual maturation followed by a subsequent decrease in mature hens. Ovoinhibitor was purified from the egg yolk plasma from nonfertilized eggs using two consecutive affinity chromatographies and gel filtration. Purified egg yolk ovoinhibitor was shown to inhibit trypsin and subtilisin. It was shown that purified egg yolk ovoinhibitor exhibited antimicrobial activities against Bacillus thuringiensis. The results suggest that this anti-protease plays a significant role in antibacterial egg defense against Bacillus spp., preventing contamination of table eggs (nonfertilized eggs) and protecting the chick embryo (fertilized eggs).
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