Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 10, Pages 5617-5624Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf104755r
Keywords
inducible expression system of protein; pSIP; beta-galactosidase; food-grade; alanine racemase; Lactobacillus
Funding
- Austrian Academic Exchange Service (OAD)
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Food-grade gene expression systems for lactic acid bacteria are useful for applications in the food industry. We describe a new food-grade host/vector system for Lactobacillus plantarum based on pSIP expression vectors and the use of the homologous alanine racemase gene (air) as selection marker. A new series of expression vectors were constructed by exchanging the erythromycin resistance gene (erm) in pSIP vectors by the L. plantarum WCFS1 air gene. The vectors were applied for the overexpression of beta-galactosidase genes from L. reuteri L103 and L. plantarum WCFS1 in an air deletion mutant of L. plantarum WCFS1. The expression levels obtained in this way, i.e. without the use of antibiotics, were comparable to the levels obtained with the conventional system based on selection for erythromycin resistance. The new system is suitable for the production of ingredients and additives for the food industry.
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