Journal
NANO LETTERS
Volume 15, Issue 11, Pages 7370-7376Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b02727
Keywords
Force propagation; single molecule force spectroscopy; steered molecular dynamics; network analysis; cohesin-dockerin
Categories
Funding
- HEG from the European Research Council [294438]
- National Institutes of Health (NIH) [9P41GM104601]
- Energy Biosciences Institute [UCB BP 2014OOO4J01]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- GIF
- German-Israeli Foundation for Scientific Research and Development
- European Union [604530]
- ERA-IB Consortium [EIB.12.022]
- Israel Science Foundation (ISF) [1349]
- United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel
- Society in Science-The Branco Weiss Fellowship program
- European Research Council (ERC) [294438] Funding Source: European Research Council (ERC)
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Here we employ single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations to reveal force propagation pathways through a mechanically ultrastable Multidomain cellulosome protein complex. We demonstrate a new combination of network-based correlation analysis supported by AFM directional pulling experiments, which allowed us to visualize stiff paths through the protein complex along which force is transmitted. The results implicate specific force-propagation routes nonparallel to the pulling axis that are advantageous for achieving high dissociation forces.
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