Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 14, Pages 7896-7901Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf201793s
Keywords
Gracilibacillus A7; alginase; oligosaccharides; root elongation; salt stress
Funding
- Tianjin Hi-tech Support Program Key Projects [11ZCGYSF01400]
- Tianjin Natural Science Foundation [09JCYBJC08800]
- Special Fund Project for Technology Innovation of Tianjin City [08FDZDSF03402]
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Alginase was purified from Gracilibacillus A7 and evaluated for its ability to produce elicitor-active oligosaccharides. The optimum conditions for the alginase reaction are as follows: temperature, 40 degrees C; pH, 8.0; alginate content, 0.3-0.7%; and the presence of Na+ and Mg2+ metal ions. The degree of polymerization (DP) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. The relative root length (RRL) of the Brassica campestris L. increased with the addition of oligosaccharides with reduced DP values. The oligosaccharides with lower DP values are effective in reducing the effect of salt stress on the activity of the superoxide dismutase (SOD) and guaiacol peroxidase (POD), and oligosaccharides with moderate DP values can reduce the increase in lipid peroiddation activities (as malondialdehyde content) induced by salt stress. These results suggest that oligosaccharides may act as osmoprotective agents during the plant germination process.
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