Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 8, Pages 3520-3531Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf104156v
Keywords
trans fatty acids; marine lipids; interferents
Funding
- Norwegian Fishery and Aquaculture Industry [900195]
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At conditions commonly applied for trans fatty analyses by gas chromatography, fatty acids naturally occurring in marine lipids may overlap chromatographically with C16 and C18 trans fatty acids and lead to false positives. Elution patterns were studied by tracking retention indices at shifting temperature conditions on two cyanopropyl-coated capillary columns. Most overlaps can be avoided by selecting the right chromatographic conditions, but it was not possible to find a single condition that eliminates the risk of overlap between trans fatty acids and interferents. In total, 17 compounds were identified as potential interferents, and the amounts of these compounds were quantified in various samples of marine origin. The interferents that will most likely contribute to incorrect assessments of trans fatty acids in marine lipids are probably 18:3 n-4 and 18:1 n-11.
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