Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 5, Pages 1646-1652Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf103290f
Keywords
Amarouciaxanthin A; PPAR gamma; C/EBP alpha; 3T3-L1; adipogenesis; GPDH activity
Funding
- MEXT (Ministry of Education, Culture, Sports, Science, and Technology of Japan)
- Regional Innovation Cluster Program (Global Type)
- MEXT [19680031]
- Grants-in-Aid for Scientific Research [19680031, 23380068] Funding Source: KAKEN
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Fucoxanthin is converted to fucoxanthinol and amarouciaxanthin A in mice. It was previously reported that fucoxanthinol attenuated the adipogenesis of 3T3-L1 cells. However, the effects of amarouciaxanthin A on adipocyte differentiation have not been clarified. This study examined the effects of amarouciaxanthin A on 3T3-L1 adipogenesis by comparing the effects of fucoxanthinol, isofucoxanthinol, and amarouciaxanthin B. Amarouciaxanthin A significantly decreased glycerol-3-phosphate dehydrogenase (GPDH) activity, which was measured as an indicator of adipocyte differentiation. The suppressive effect of amarouciaxanthin A was stronger than that of fucoxanthinol, amarouciaxanthin B, and isofucoxanthinol. The mRNA expressions of adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and glucose-transporter 4 (Glut4) in 3T3-L1 cells were markedly down-regulated by amarouciaxanthin A compared to fucoxanthinol. Furthermore, the expression levels of peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT-enhancer-binding protein alpha (C/EBP alpha) which are the key adipogenic transcriptional factors, were also de creased by amarouciaxanthin A during adipocyte differentiation. These results show that amarouciaxanthin A, which is a dominant metabolite of fucoxanthin in white adipose tissue, suppressed 3T3-L1 adipocyte differentiation.
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