Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 5, Pages 3167-3176Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf903841k
Keywords
Mediterranean propolis; GC-MS profiling; diterpenes; mass spectra; retention indices
Funding
- Nalional Science Fund
- Ministry of Education and Sciences of Bulgaria [PD-3/2007]
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The objective of this work is to analyze and identify the diterpene compounds in Mediterranean propolis samples from different Greek regions by GC-MS. The chemical composition of six propolis samples was established using previously isolated diterpenes from Cretan propolis as authentic standards for identification, based on mass spectral fragmentation of the TMS derivatives and retention index. More than 30 diterpenes, among which were new propolis constituents, were identified and characterized by means of authentic standards and interpretation of MS fragmentation as well. This is the first detailed profiling of a new type of propolis, rich in diterpenes, The chromatographic and mass- spectral characteristics of the diterpenes identified could be very useful for rapid GC-MS profiling of this propolis type and for revealing its plant sources.
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