Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 7, Pages 4097-4103Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf9032377
Keywords
alpha-Glucosidase inhibitor; Bacillus subtilis B2; purification; fermented okara; 1-deoxynojirimycin
Funding
- Japan International Research Center for Agricultural Sciences (JIRCAS)
- 11th 5-year Plan of the People's Republic of China [2006BAD27B09]
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This study was to purify an alpha-glucosidase inhibitor from okara (soy pulp) fermented by Bacillus subtilis B2 and to identify its chemical structure. Membrane dialysis, active charcoal, CM-Sepharose chromatography, and preparative thin-layer chromatography (TLC) were used in the purification, while positive mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry were used in the identification. The MS and NMR data showed that the purified alpha-glucosidase inhibitor was 1-deoxynojirimycin (DNJ) with a molecular weight of 163 Da. This is the first time that DNJ was isolated from foods fermented with Bacillus species. Okara fermentation with B. subtilis B2 might be used to produce a food-derived DNJ product as a functional food for diabetic patients.
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